Expression of bone morphogenetic protein 2, 4, and related components of the BMP signaling pathway in the mouse uterus during the estrous cycle     

Yan Li  Quan-wei Wei  Jian-gang Feng  Mu-lin Xu  Rui-hua Huang  Fang-xiong Shi 《Journal of Zhejiang University. Science. B》2014,15(7):601-610

The objective was to investigate the expression of bone morphogenetic protein (BMP) family members in the mouse uterus during the estrous cycle by real-time polymerase chain reaction (PCR) and immunohistochemistry. Uterine samples from Swiss ICR mice were collected and dissected free of surrounding tissue. One uterine horn was snap frozen in liquid nitrogen immediately after collection and stored at −80 °C for RNA extraction, and the other was fixed in 40 mg/ml paraformaldehyde at room temperature for immunolocalization of BMP2 protein. Real-time PCR analysis showed that the expression level of Bmp2 was significantly higher at proestrus than at estrus and metestrus (P<0.05). The relative abundance of Bmp4 exhibited significant fluctuations, but there were no statistically significant differences between the expression levels of Bmp2 and Bmp4 (P>0.05). The expression levels of Bmpr1a and Bmpr2 remained unchanged during estrous cycles. However, the level of Bmpr1b mRNA decreased significantly at estrus (P<0.05), increasing subsequently at metestrus. Furthermore, the level of Bmpr1b mRNA was significantly lower than those of Bmpr1a and Bmpr2 mRNA at the corresponding stages (P<0.05). All three receptor-regulated Smads (R-Smads) detected were differentially expressed in the mouse uterus and the expression levels of Smad1 and Smad5 were significantly higher than that of Smad8 (P<0.05). In addition, the expression level of Smad4 did not change substantially throughout the estrous cycle. Immunohistochemical experiments revealed that BMP2 protein was differentially expressed and localized mainly in the uterine luminal and glandular epithelial cells throughout the estrous cycle. In conclusion, our results provide information about the variation in the mRNA levels of Bmp2 and Bmp4 and related components of the BMP signaling pathway. The data provide quantitative and useful information about the roles of endometrial BMP proposed and demonstrated by others, such as the degradation and remodeling of the endometrium.  相似文献   

不同低氧训练方式对血脂、体重及其变化机理的研究     

马延超  张缨  刘花层 《中国体育科技》2007,43(5):136-140

观察不同低氧训练方式对血清瘦素、下丘脑神经肽Y、下丘脑瘦素受体、血脂及其体重等指标的影响,探求低氧训练影响机体血脂、体重变化的机理。实验对象:SD大鼠50只,随机分为高住高训组(HiHi)、高住低训组(HiLo)、高住对照组(HiCo)、低训对照组(LoCo)和低住低训组(LoLo),进行4周不同低氧状态下的游泳训练。低氧环境的氧浓度为12.7%(相当于海拔4000m)。实验方法:血清leptin和下丘脑NPY用放射免疫分析法测定,血脂用全自动生化分析仪测定,下丘脑leptin受体采用放射配体检测法测定。结果:1)低氧暴露4周血清TC、TG、HDL、LDL升高;2)低氧训练4周,血清TC、HDL、TG、LDL降低,但HiLo组降低血清TG作用较HiHi组显著;3)低氧暴露和低氧训练4周,均观察到血清Leptin、下丘脑NPY有下降趋势;4)低氧暴露和低氧训练4周,下丘脑K1、K2没有变化,但下丘脑q1、q2分别增大。结论:1)低氧暴露4周可能有升高血脂的作用;2)低氧训练4周血脂可能降低;3)低氧暴露或低氧训练4周,下丘脑Leptin受体结合容量可能增大,使下丘脑NPY的分泌下降,抑制体重的增长。  相似文献   

透过传播学原理看隐性广告     

王轶群 《宁波大红鹰职业技术学院学报》2007,(2)

近几年来,隐性广告越来越多地出现在我们的视野中,作为一种新兴的广告形式,隐性广告在一定程度上改变了原有广告的传播模式,收到了良好的传播效果。这一现象也引发了众多业内人士与专家学者的讨论研究。隐性广告自身的特点决定了它受众群体的接受层次和范围,正确利用好这个传播的形式才能将其传播效果最大化。  相似文献   

雄激素受体在低氧和运动条件下对骨骼肌蛋白质合成的作用     

叶鸣  贺道远  曾凡星 《体育学刊》2008,15(12)

雄激素促进骨骼肌蛋白质合成是通过雄激素受体作用的，高原训练时高原低氧抑制蛋白质合成，导致肌肉质量下降。综述了雄激素受体的结构，作用机理，在骨骼肌中的作用机制，低氧和运动对雄激素受体的作用，明确低氧和运动条件下雄激素受体在骨骼肌蛋白质合成中的可能作用机理。  相似文献   

小灵猫红细胞免疫功能的研究     

张德成  陈思义 《科技通报》1991,7(4):231-233

本文采用免疫花环试验法测定了健康小灵猫红细胞上C_(3b)受体和免疫复合物的数量33例。红细胞上C_(3b)受体花环率的均值为9.21±1.81;免疫复合物花环率的均值为5.50±1.36。这一研究结果充平表明,小灵猫的红细胞不但具有携氧、运输气体等功能外,而且还具有重要的免疫功能。  相似文献   

Lipid Peroxidation Correlates with HIVmRNA in Serodiscordant Heterosexual HIVpartners of Nigerian Origin     

Ibeh BO  Obidoa O  Nwuke C 《Indian journal of clinical biochemistry : IJCB》2011,26(3):249-256

We recruited 59 individuals of known HIV serostatus after informed consent however, 44 were serodiscordant heterosexual partners [serodiscordant seronegative (SSN group) and serodiscordant seropositive (SSP group)] while 15 were seronegative healthy individuals (SNH). In the case–control study we choose to determine Malondialdehyde (MDA) concentration as a marker of lipid peroxidation index (oxidative stress) spectrophotometrically and quantify HIV mRNA by Real Time-nucleic acid sequence based amplification assay (RT-NASBA). Here our result show for the first time a high concentration of lipid peroxidation product (MDA, 116.6%) with a significant (P < 0.05) increase in HIV serodiscordant seropositive subjects over their seronegative partners. However, Spearman rank correlation statistics of SSP group showed a positive correlation value (P < 0.01, r = 0.89) between MDA and mRNA and a negative correlation between MDA and T-cell ratio (P < 0.01, r = 0.96).The study may strongly indicate a possible lipid peroxidation product threshold for predicting HIV infection and progression in serodiscordant heterosexual partners.  相似文献   

用非放射性标记法研究低密度脂蛋白受体     

楼定安  朱伟民 《科技通报》1994,10(3):137-141

低密度脂蛋白受体（ＬＤＬ－Ｒ）的缺陷是动脉粥样硬化的重要危险因素之一，检测ＬＤＬ－Ｒ对高胆固醇血症的鉴别诊断和治疗具有重要意义，本文总结本实验室多年来应用各种非放射性标记法（如荧光素、过氧化物酶、生物素）或免疫检测法代替经典的放射性配体法研究ＬＤＬ－Ｒ的经验，并推荐在临床检验中以生物素化ＬＤＬ为配体测定外周血单个核细胞ＬＤＬ－Ｒ（即细胞－ＢＡ－ＥＬＳＡ），较为简便、灵敏、经济、实用．  相似文献   

Examining the link between emotional childhood abuse and social relationships in midlife: The moderating role of the oxytocin receptor gene     

《Child abuse & neglect》2019

BackgroundA single nucleotide polymorphism (SNP), rs53576, of the oxytocin receptor gene (OXTR) has been associated with fundamental aspects of social processes and behaviors. Compared to A carriers, GG individuals have enhanced social competencies and tend to elicit more positive responses from social partners. However, the G allele of the OXTR rs53576 has also been associated with greater social sensitivity.ObjectiveThe current study examined the unique influence of emotional childhood abuse on positive and negative aspects of different types of social relationships (e.g., family, spouse/partner, and friends) in midlife and whether genetic variations of OXTR rs53576 moderated these associations.Participants and Setting: A total of 614 participants in midlife (aged 40–65), recruited for a large-scale study of Phoenix metropolitan residents (AS U Live Project), answered self-report questionnaires and provided blood samples for DNA genotyping.MethodsHierarchical multiple regression analyses tested whether emotional childhood abuse predicted social support and strain for each relationship type and whether these potential linkages differed by OXTR genotype (GG versus AA/AG).ResultsOverall, individuals with a history of emotional childhood abuse had less supportive and more strained relationships in midlife. For supportive family relationships, the effect of emotional childhood abuse was moderated by OXTR rs53576 (p < .001). Under conditions of experiencing more emotional abuse in childhood, GG individuals had more supportive family relationships in midlife compared to A carriers.ConclusionsOverall, genetic variations of OXTR rs53576 may be an important candidate in understanding the development of social functioning within the context of emotional childhood abuse.  相似文献   

“That which we call a rose by any other name would smell as sweet”: How the nose knows!     

Sayanti Saha  Parvathy Ramakrishnan  Sandhya S Visweswariah 《Resonance》2005,10(3):28-34

Richard Axel and Linda B Buck received the Nobel Prize for their discoveries leading to an understanding of how odors are perceived. A large family of receptors present in the nasal epithelium are activated by specific odorants and relay information on the signal to the brain, allowing us to have the sense of smell.  相似文献   

肥胖影响健康的分子生物学基础   总被引：2，自引：0，他引：2  

代天修 《体育成人教育学刊》2007,23(4):55-56

白色脂肪组织能分泌瘦素、脂联素、抵抗素、肿瘤坏死因子-α、白细胞介素-6等激素与细胞因子。肥胖者白色脂肪组织含量增加,改变了这些激素与细胞因子的水平,从而影响健康。  相似文献